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- Reference Material for Genetic Testing (DNA Reference Material)
- Infectious diseases
Reference Material for Genetic Testing (DNA Reference Material)
Infectious diseases
Issues associated with PCR testing
- Can you say for certain that a person is not infected with the virus if they have a negative test result?
- 从一个设备到anot不一致的性能her
- Inconsistent performance depending on what kind of equipment and reagents are used
DNA reference plate that overcomes issues associated with PCR testing
DNA在whi参考板产品ch DNA comes dispensed into a PCR plate or tube well in 1-copy units. It is used in reaction systems (measurement systems, detection systems) that can be difficult to work with due to the limitations of the dilution method or evaluating reagent performance in low-concentrations.
- Can be used to quality control PCR tests.
- correctly assess the performance of LDT (Laboratory Developed Test) reagents.
How the DNA reference plate is made
Homologous recombination
- A specific DNA sequence*is intro-duced into a cell by homologous recombination
*A base sequence in the target detection zone in genetic testing
Precise cell dispensing
- Dispensed using inkjet technology at the same time as counting the number of dispensed cells
DNA reference plate
- ・A plate containing a specific DNA sequence with the desired number of copies in each well
- ・A plate with a low-concentration DNA molecule count
- ・A plate where the absolute DNA count is predetermined
By combining homologous recombination technology with Ricoh’s own precise cell dispensing technology based on inkjet, it has been possible to manufacture DNA reference plates that contain a predetermined number of copies of the target DNA sequence.
The use of DNA reference plates
A DNA reference plate can be used in quality control in order to create calibration curves and to evaluate detection limits, etc. Simply perform PCR measurement using the DNA reference plate before performing PCR tests using the sample.
【Step1】
Purchase the DNA reference plate
【Step2】
PCR preparation
【Step3】
PCR
Quality control is possible even with low-concentrations, down to a single copy.
Results measuring novel coronavirus SARS-CoV-2 (N gene)
Measurement using
1, 2, 4, 8, and 16 copies
(each n = 16 measurements)
Measurement using
1, 5, 10, 50, and 100 copies
(each n = 16 measurements)
Given the limitations with the conventional dilution method, variations occur when DNA is in concentrations of less than 100 copies, so it can be difficult to create a highly reproducible calibration curve or measure detection limits. With a DNA reference plate, where the DNA count can be predefined in 1-copy units, quality control can be performed with good replicability even in low concentrations.
Results measuring norovirus (GI, GII gene)
DNA sequences of norovirus
Introduce part of the viral DNA
into the genome of the cell
We plotted the mean Cq values (n = 4 measurements) from qPCR testing. Results where fewer than 100 copies were dispensed used inkjet technology, and 1,000 copies or more, used yeast genome extraction products.
Acquisition process
This product is sold for use in all types of research. It cannot be used in medical treatment and diagnosis.
Thank you for your interest in the RICOH Standard DNA Series.
For any other information, please contact us via email at standard_dna_series@jp.ricoh.com